Abstrato

Biochemical study of combined and separated encapsulated rivaroxaban and ubiquinone liposomal nanoparticles and some herbals on the myocardial infracted rat model

AbouZaid OAR, Abulyazid I, Ashour OH & EL-din Zohni MS

Background: Anthracyclines such as doxorubicin are highly effective chemotherapy agents used to treat many common malignancies. However, their use is limited by cardiotoxicity. Rivaroxaban, a direct Factor Xa inhibitor, is now approved in United States and elsewhere for the prevention of venous thrombo-embolism. Coenzyme Q is mitochondrial respiratory chain element. Ginger has staring potential for treating cardiovascular disorders (atherosclerosis and hypertension). Murraya koenigii is known as ‘curry patta’ has anti-oxidant, anti-tumor, anti-inflammatory effects. Aim of study: In order to evaluate the protective effect of rivaroxban, ubiquinone separated and combined in the form of liposomes, combined curry and ginger on the myocardial infarcted rat model induced by doxorubicin. In vivo study was carried out, free doxorubicin; different liposomal preparations were assessed by different measurements. Materials and methods: The tested groups received respectively, group (1) Control. (2) Group B:received doxorubicin. (3) Group Q: liposomal ubiquinone and doxorubicin.(4) Group R: liposomal rivaroxban and doxorubicin. (5) Group QR: liposomal rivaroxban and ubiquinone encapusulated on the same liposome and doxorubicin. (6) Group D: blend of ginger, curry and doxorubicin. Particles size, zeta potential and TEM were measured for liposomal preparations. Several parameters have been measured to assess the cardiotoxicity (Albumin, lactate dehydrogenase enzyme (LDH), Aspartate Aminotransferase (AST), Anticardiolipin IgG, Creatine kinase-MB (CK MB), C-reactive protein (CRP), Tumor necrosis factor (TNF α)) and histopathological studies. Results: Rats with free doxorubicin caused significantly elevated levels of all parameters measured compared to control group, contol group vs. dox. treated group was as follow at p<0.05 (albumin 4.435 ± 0.085 vs. 2.910 ± 0.10, anticardiolipin 9.4 ± 0.256 vs.19.31 ± 0.4391, AST 64.60 ± 1.79 vs. 244.8 ± 9.86, CK 14.04 ± 1.794 vs. 73.25 ± 1.240, CRP4.904 ± 0.45 vs. 23.96 ± 0.95, LDH 212 ± 10.1 vs. 754 ± 16.4, TNF α 34.62 ± 1.05 vs.126.6 ± 6.25). In addition there is a significant variation of other groups compared to group B. Conclusionc There is a marked cardiotoxicity of doxorubicin could be decreased by the administration of antithrombic rivaroxban, respiratory chain element ubiquinone, herbals like ginger and curry.