Abstrato

Noncoding RNAs, post-transcriptional RNA operons and Chinese hamster ovary cells

Fabian Stiefel, Simon Fischer, Matthias Hackl, Rene Handrick, Friedemann Hesse, Nicole Borth, Kerstin Otte and Johannes Grillari*

Chinese hamster ovary cells are among the biotechnologically most relevant production systems for biopharmaceuticals. With the availability of the genomic sequences of Chinese hamster, rational systems biotechnology-driven approaches to optimize this cell factory have become available. Here, we review the current status of noncoding RNAs as members of the post-transcriptional operon concept in the context of Chinese hamster ovary cell line engineering and bioprocessing. In addition, we suggest that they already now keep their promises as tools for increasing specific productivity and thus time space yield of biopharmaceutical production, a feature that will allow for more cost efficient production processes in the future.